MerFISH

Multiplexed Error-Robust Fluorescence in situ Hybridization

MerFISH instrumental setup at DaMBIC

MerFISH – Imaging-based Spatial Transcriptomics Platform

• Advanced platform for spatially resolved transcriptomics analysis.
• Compatible with a wide range of cell cultures and tissue samples, including formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissues.
• High-plex gene expression analysis, capable of profiling hundreds to thousands of RNA species simultaneously. Customizable panels to target specific genes of interest, providing flexibility for diverse research needs.
• Flexible microscope platform allowing different levels of detail, depending on the application and need (resolution down to ~150 nm).

Explore the spatial transcriptomics capabilities with MerFISH

MerFISH combines the principles of fluorescence in situ hybridization (FISH) with multiplexed error-robust barcoding strategies. Sequential hybridization of probe sets, followed by imaging and signal decoding, allows for the precise identification of RNA species in a multiplexed manner.

The MerFISH workflow. Source: Chen, Kok Hao, et al. 2015. "Spatially resolved, highly multiplexed RNA profiling in single cells." Science.

The versatility and adaptability of MERFISH makes it a valuable tool for uncovering the complex molecular landscape of cells and tissues. See, among others, the following publications:

• Mapping disease-related changes in gene expression: Cadinu, Paolo, et al. 2024. Cell. https://www.cell.com/cell/fulltext/S0092-8674%2824%2900254-X
• Characterize subcellular compartmentalization of RNAs: Xia, Chenglong, et al. 2019. PNAS. https://www.pnas.org/doi/abs/10.1073/pnas.1912459116

How to get access to the MerFISH technique?

For access to the MerFISH technique, under the CellX platform, please reach out to the Danish Molecular Biomedical Imaging Center (DaMBIC).

Location: V10-602a-0 (merfish)