Nikon Ti-2 and Ti Eclipse Widefield

DaMBIC provides two inverted widefield microscopes; a Nikon Ti-2 Eclipse and a Nikon Ti Eclipse.

Nikon Ti-2 Eclipse

2020-08-04 11.08.04

  • LED-illumination (CoolLED pE-300, UV, blue, green) for epifluorescence excitation. LED diascopic transmission illumination.
  • sCMOS camera, Andor Zyla (5.5 MP, 2560 x 2160, 30 fps, 11 bit and 16 bit data range)
  • Motorized stage for automated/smart sample exploration.
  • Filter cubes: DAPI, FITC, TRITC, CY5.
  • Objectives: 10x/0.45 air, 20x/0.75 air, 60x/1.20 water, 60x/1.40 Oil, 20x/0.40 LWD Ph1.
  • The microscope PC is installed with Nikon NIS-Elements that is also used on other DaMBIC microscopes allowing easy familiarization with the user environment across the different microscopes. Besides basic image acquisition, many applications can be accessed such as large image acquisition, multidimensional imaging (X, Y, Z, time, lambda, multipoint).
  • Applications:
    • Widefield epiflourescence microscopy.
    • Widefield (bright-field) transmission microscopy.
    • Phase contract microscopy.
    • Automated Multi-Dimensional acquisition (xyz, time, lambda, magnification, large image acquisition).
    • Easy sample overview/navigation by automated exploration of entire sample.
  • Nikon Ti-2 flyer.
  • NIS Elements flyer
  • Location: V10-602a-0

Nikon Ti Eclipse

  • LED-illumination (UV, blue, green) for epifluorescence excitation
  • Halogen lamp for transmission microscopy
  • sCMOS camera
  • Motorized stage for automated/smart sample exploration.
  • Filter cubes: DAPI, FITC, TRITC, CY5.
  • Objectives: 10x air, 20x air, 40x air, 60x Oil, 60x Water.
  • Applications:
    • Widefield epiflourescence microscopy
    • Widefield (bright-field) transmission microscopy
    • Phase contract microscopy
    • Automated Multi-Dimensional acquisition (time, illumination, magnification, position)
    • Easy sample overview/navigation by automated exploration of entire sample.
  • Protocol for microscope usage Nikon wide field protocol.pdf
  • Location: V12-604-0
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