Stochastic Optical Reconstruction Microscopy (STORM) reconstructs a super-resolution image by combining precise localization information for individual fluorophores in microscope specimens. This multi-color localization data in three dimensions (xyz) generates super-resolved images at 10 x the resolution of conventional light microscopes (up to 20 nm in x,y, 50 nm in z). This enables visualization of molecular interactions and organizations on the nanoscale.
Acquisition times down to the ~10 seconds-range per frame is possible (high-speed mode, 20 µm x 20 µm area).
Camera ORCA-Flash 4.0 sCMOS camera (Hamamatsu). Field of view is up to 80 µm x 80 µm.
The LU-NV laser unit contains the following lasers: 405 nm, 488 nm, 561 nm, 647 nm.
The Nikon N-SIM is build onto a Ti-2 LFOV microscope body available with the following objectives: 100x Oil, 100x Si.
NB. operating conditions are restricted to 20-25°C (+/- 0.5°C) i.e. temperature fluctuations are not tolerated well.
The microscope PC is installed with Nikon NIS-Elements that is also used on other DaMBIC microscopes allowing easy familiarization with the user environment across the different microscopes.
Overview of the Nikon super resolution microscopes (SIM and STORM).