Leica SP8 CARS and STED
Leica SP8 microscope combines possilibty to perform Stimulated emission depletion microscopy (STED), Coherent anti-Stokes Raman spectroscopy (CARS) and fluorescent confocal microscopy.
Flexible excitation (470-700 nm), filter-free spectral detection, simultaneous 3-colour detection, Super-resolution STED at 592 nm (green dyes), resonant scanners, gated detection.
Confocal: The system is a standard laser scanning confocal microscope. Excitation: The system is equipped with a white light laser (WLL) for a flexible choice of up to eight simultaneous excitation wavelengths (470-700 nm). The system further has a choice for argon laser excitation at 458 nm, 476 nm, 488 nm, 496 nm, and 514 nm. Detection: Emission wavelength collection is flexible because of the filter-free spectral detection system. The system is equipped with three point detectors; two PMT detectors and one hybrid (HyD) detector. The HyD detector has gated detection. Objectives: 10X/0.4 dry, 20X/0.75 IMM, 40X/1.1 water, 63X/1.4 oil, 100X/1.4 oil.
Further features: The system features eg. multiple ROI selection, scan speeds up to 7 frames/s (512x512 pixels), resonant scanner (8 kHz) with scan speeds of up to 28 frames/s (512 x 512 pixels), xyz, xzy, xyzt, xzyt, and lambda scan modes (both excitation and emission).
STED: The STED (STimulated Emission Depletion) systems is used with the above confocal system. Depletion: The system is equipped with a 592 nm continuous wave (CW) STED laser allowing for super-resolution microscopy. Sub-diffraction limited spatial (xy) resolution of down to ~50 nm standard green emitting STED dyes (eg. alexa488, Atto-488, abberior star 488, chromeo488). Objective: 100X/1.4.
CARS system: laser 1064nm, objectives: 20x Air (IMM) or 40x IRAPO water.
Manual for microscope usage Leica STED-CARS-manual.pdf
Software for data analysis can be found here Leica LAS X